I had the problem, that I was using an jquery ajax request on a website. I was using the document ready to initialize the function. When a .NET ajax request happend afterwards, the functionality of my jquery was not enabled anymore.
Solution of that was, to initialize the function to the Sys.WebForms.
It worked like that:
jQuery(document).ready(function () {
Myfunction();
load();
});
function load() {
Sys.WebForms.PageRequestManager.getInstance().add_endRequest(Myfunction);
}